Journal: bioRxiv
Article Title: Evidence for a RIPK1-independent survival mechanism for CASPASE-8 in αβ T cells
doi: 10.1101/2025.03.27.645705
Figure Lengend Snippet: (A) Bar charts are MFI of CD5 expression normalised to the average level in Cre -ve T cells of the indicated naive subsets. (B) Histograms are of IL-7R expression by naive CD4 and CD8 T cells from the indicated donors, and bar charts are summary of expression normalised to average MFI of Cre -ve controls. (C) Gating strategy to track the indicated donor populations in CD45.1 + CD45.2 + hosts amongst naive CD4 and naive CD8 populations in blood. (D) Line graphs summarise representation of donor populations amongst total naive CD4 and naive CD8 populations in blood, and (E) their expression of IL-7R, normalised to average MFI of host subsets. Data are representative of two independent experiments.
Article Snippet: Panels used the following mAb: PE-Cy7-conjugated antibody against CD25 (eBioscience), PE-conjugated antibody against CD127 (ThermoFisher Scientific), BV785-conjugated CD44 antibody (Biolegend), BV421-conjugated antibody against CD4 (Biolegend), BUV395-conjugated antibody against CD8 (BD Biosciences), BV711-conjugated antibody against CD24 (BD Biosciences), PerCP-Cy5.5-conjugated antibody against TCR (Cambridge Biosciences), FITC-conjugated antibody against CD5 (eBiosciences), BV650-conjugated antibody against CD45.1 (Biolegend), FITC-conjugated antibody against CD45.2 (ThermoFisher Scientific), APC-conjugated antibody against CD49d (Biolegend), FITC-conjugated antibody against Ki67 (ThermoFisher Scientific).
Techniques: Expressing